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Herpes Simplex and Varicella Zoster Viruses

Fast, Efficient, and Accurate Processing for PCR Detection



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Is Extraction Required for Dermal and Anogenital Specimens?

Slide 5

December 2009

First, we needed to examine why extraction was done for HSV and VZV PCR testing. We decided to focus specifically on dermal and anogenital samples, since these comprise the bulk of the samples that we receive for HSV and VZV PCR testing.

In these cases, the main purpose of extraction is to purify viral DNA remove inhibiting substances. Since HSV and VZV DNA is typically abundant in anogenital and dermal samples, and samples collected on a swab contain very few, if any, inhibiting substances, we hypothesized that extraction served little purpose, other than removing the phenol red pH indicator from the M5 media. We know that the phenol red can interfere with the detection of the PCR product, but if samples are not submitted in M5, then extraction may not be necessary.

Required Extraction

 


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