Herpes Simplex and Varicella Zoster Viruses
Fast, Efficient, and Accurate Processing for PCR Detection
The Viruses
December 2009
To begin, herpes simplex virus, otherwise known as HSV, and varicella zoster virus, or VZV, are 2 common viruses in the herpesviridae family. They both can cause lesions of the skin and mucosal membranes: 2 common manifestations of HSV infection are oral herpes or cold sores, and genital herpes, while VZV causes chicken pox and shingles. Rarely, HSV and VZV can also cause systemic disease, but dermal and anogenital disease are much more common. The classic presentations of these diseases can usually be diagnosed without laboratory testing, but there are also situations in which the viruses have overlapping clinical manifestations, and laboratory testing may be indicated in these instances.
The Viruses |
Jump to section:
- Introduction
- The Viruses
- Laboratory Diagnosis
- HSV/VZV Real-time PCR Sample Workflow
- Is Extraction Required for Dermal and Anogenital Specimens?
- Direct Processing in Neutralization Buffer: An Alternative to MagNA Pure Extraction
- Match Up Identifying Information
- Remove Swab from the Culturette Tube
- Heat Swab in Neutralization Buffer
- Sample is Ready for PCR
- A New Workflow for Dermal and Anogenital Swabs
- Direct Processing in Neutralization Buffer: Comparable to DNA Extraction?
- Comparison of Detection - HSV
- Results: Comparison of Amplification Cycle Threshold
- Comparison of Detection - VZV
- Results: Comparison of Amplification Crossing Points
- Questions and Answers
- Questions and Answers
- M5 Media vs Culturette Tubes: Experiment
- M5 Media vs Culturette Tubes: Results
- Conclusions
- Questions?
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