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Herpes Simplex and Varicella Zoster Viruses

Fast, Efficient, and Accurate Processing for PCR Detection



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Questions and Answers

Slide 18

December 2009

Another question that you may have already thought of is whether or not there is an advantage to using M5 media for maintaining detectable DNA during transport. Clearly, we wouldn’t want to recommend the culturette tube for transport if the M5 media provided a significant advantage.

Well, it turns out that M5, while an excellent transport media for viral culture, is not actually beneficial for PCR detection of HSV and VZV from dermal and anogenital swabs. In fact, it is better to submit the sample in a culturette tube, because the virus does not need to be viable in order for DNA to be detected, and the addition of M5 media may dilute the sample, and decrease the sensitivity of detection.

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