Herpes Simplex and Varicella Zoster Viruses
Fast, Efficient, and Accurate Processing for PCR Detection
Results: Comparison of Amplification Crossing Points
December 2009
The crossing points with the NB method were also equal or lower than those using the M5 extraction methods, again showing that the NB method is at least equal to the M5 extraction method yielding amplifiable VZV DNA.
Results |
Jump to section:
- Introduction
- The Viruses
- Laboratory Diagnosis
- HSV/VZV Real-time PCR Sample Workflow
- Is Extraction Required for Dermal and Anogenital Specimens?
- Direct Processing in Neutralization Buffer: An Alternative to MagNA Pure Extraction
- Match Up Identifying Information
- Remove Swab from the Culturette Tube
- Heat Swab in Neutralization Buffer
- Sample is Ready for PCR
- A New Workflow for Dermal and Anogenital Swabs
- Direct Processing in Neutralization Buffer: Comparable to DNA Extraction?
- Comparison of Detection - HSV
- Results: Comparison of Amplification Cycle Threshold
- Comparison of Detection - VZV
- Results: Comparison of Amplification Crossing Points
- Questions and Answers
- Questions and Answers
- M5 Media vs Culturette Tubes: Experiment
- M5 Media vs Culturette Tubes: Results
- Conclusions
- Questions?
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