Herpes Simplex and Varicella Zoster Viruses
Fast, Efficient, and Accurate Processing for PCR Detection
Comparison of Detection - VZV
December 2009
We also assayed 100 known positive VZV samples using the two methods. As you see here, 12 positive samples were detected by both methods, and a single additional positive sample was detected by the NB method. This demonstrates that the NB method is at least equivalent to the M5 extraction method.
Comparison of Detection |
Jump to section:
- Introduction
- The Viruses
- Laboratory Diagnosis
- HSV/VZV Real-time PCR Sample Workflow
- Is Extraction Required for Dermal and Anogenital Specimens?
- Direct Processing in Neutralization Buffer: An Alternative to MagNA Pure Extraction
- Match Up Identifying Information
- Remove Swab from the Culturette Tube
- Heat Swab in Neutralization Buffer
- Sample is Ready for PCR
- A New Workflow for Dermal and Anogenital Swabs
- Direct Processing in Neutralization Buffer: Comparable to DNA Extraction?
- Comparison of Detection - HSV
- Results: Comparison of Amplification Cycle Threshold
- Comparison of Detection - VZV
- Results: Comparison of Amplification Crossing Points
- Questions and Answers
- Questions and Answers
- M5 Media vs Culturette Tubes: Experiment
- M5 Media vs Culturette Tubes: Results
- Conclusions
- Questions?
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