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Herpes Simplex and Varicella Zoster Viruses

Fast, Efficient, and Accurate Processing for PCR Detection


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Results: Comparison of Amplification Cycle Threshold

Slide 14

December 2009

We also examined the cycle thresholds or crossing points of the PCR amplification curves. The lower the crossing point, the higher the amount of viral DNA in the sample. Shown here are just the results from the first eight samples, but it is enough to show that the crossing points are consistently lower using the NB method compared to the M5 extraction method. And this trend continued throughout all 300 samples, demonstrating that the NB method resulted in a great yield of amplified HSV DNA.



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