Herpes Simplex and Varicella Zoster Viruses

Results: Comparison of Amplification Cycle Threshold

December 2009

We also examined the cycle thresholds or crossing points of the PCR amplification curves. The lower the crossing point, the higher the amount of viral DNA in the sample. Shown here are just the results from the first eight samples, but it is enough to show that the crossing points are consistently lower using the NB method compared to the M5 extraction method. And this trend continued throughout all 300 samples, demonstrating that the NB method resulted in a great yield of amplified HSV DNA.

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Jump to section:

• Introduction
• The Viruses
• Laboratory Diagnosis
• HSV/VZV Real-time PCR Sample Workflow
• Is Extraction Required for Dermal and Anogenital Specimens?
• Direct Processing in Neutralization Buffer: An Alternative to MagNA Pure Extraction
• Match Up Identifying Information
• Remove Swab from the Culturette Tube
• Heat Swab in Neutralization Buffer
• Sample is Ready for PCR
• A New Workflow for Dermal and Anogenital Swabs
• Direct Processing in Neutralization Buffer: Comparable to DNA Extraction?
• Comparison of Detection - HSV
• Results: Comparison of Amplification Cycle Threshold
• Comparison of Detection - VZV
• Results: Comparison of Amplification Crossing Points
• Questions and Answers
• Questions and Answers
• M5 Media vs Culturette Tubes: Experiment
• M5 Media vs Culturette Tubes: Results
• Conclusions
• Questions?