Diagnosis and Classification of Amyloidosis
Subtyping in Tissues
December 2009
For typing of amyloidosis in paraffin-embedded tissues, antibody-based assays using immunohistochemistry have been the gold standard but they have low specificity and sensitivity. This is due to a number of reasons including epitope loss in the physical structure of the amyloid and increased background to nonspecific reactivity. Moreover, immunohistochemistry tests are often not validated specifically for diagnosis of amyloidosis. The interpretation is based on comparison of intensity between independent tests. The tests are not quantitative and, importantly, antibody assays require prior knowledge of possible targets.
Subtyping in Tissues |
Jump to section:
- Introduction
- Amyloidosis
- Diagnosis1
- Slide Images
- Subtypes: Historical Context
- Subtypes2
- Subtyping
- Subtyping
- Subtyping in Tissues
- Mass Spectrometry-Based Proteomic Analysis of Amyloidosis
- Protein Extraction
- Fragmented Peptides
- Mass/charge of Daughter Ions Measured
- Bioinformatics
- Case History 1
- Bone Marrow Biopsy Image
- Flow Cytometry Immunophenotyping
- Bone Marrow Image
- Congo Red-Positive Slide Image
- Immunohistochemistry Slide Images
- Microdissection
- MS-Based Proteomic Analysis
- Diagnosis
- Case History 2
- Mass Spectrometry Results in 50 Cases of Amyloidosis3
- Summary: Diagnosis
- Summary: Classification
- References
- Questions?
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