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Diagnosis and Classification of Amyloidosis



Subtyping in Tissues

Slide 9

December 2009

For typing of amyloidosis in paraffin-embedded tissues, antibody-based assays using immunohistochemistry have been the gold standard but they have low specificity and sensitivity. This is due to a number of reasons including epitope loss in the physical structure of the amyloid and increased background to nonspecific reactivity. Moreover, immunohistochemistry tests are often not validated specifically for diagnosis of amyloidosis. The interpretation is based on comparison of intensity between independent tests.  The tests are not quantitative and, importantly, antibody assays require prior knowledge of possible targets.

Subtyping in Tissues

 


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