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Diagnosis and Classification of Amyloidosis



Summary: Classification

Slide 30

December 2009

Once the diagnosis of amyloidosis is established, it is essential to facilitate tissue-based typing. As we discussed, this could be done by a number of methods. Immunofluorescence/antibody based assays on frozen tissue provide good sensitivity and specificity for amyloid typing but require fresh/frozen tissue restricting their use in routine diagnosis beyond renal pathology.

In contrast, immunohistochemistry-based assays are readily applicable on most routine paraffin-embedded biopsy specimens but provide low sensitivity and specificity in even the most experienced laboratories. For these reasons, in our practice we recommend mass spectrometry- based proteomic analysis as the main method for amyloid typing.

As always, it is also very important to correlate the mass spectrometry findings with the clinical features to establish the best management approach.

Summary: Classification

 


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