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Diagnosis and Classification of Amyloidosis


Slide 3

December 2009

Diagnosis of amyloidosis is made by examination of biopsy specimen with special stains which give a characteristic reaction based on the physical structure of the amyloid. Of these, Congo red histochemical staining is believed to be the most sensitive and specific method. For best results, Congo red staining should be performed on 10 micron sections and viewed under a strong light source using either polarizing or fluorescent filters. Other, somewhat less specific stains or methods widely used for diagnosis of amyloidosis include thioflavin and sulphated alcian blue histochemical stains, immunohistochemistry against serum amyloid P component (SAP) and electron microscopy.



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