Diagnosis and Classification of Amyloidosis
Mass Spectrometry Results in 50 Cases of Amyloidosis3
December 2009
To establish the clinical sensitivity and specificity of mass spectrometry based proteomic analysis in amyloid classification, we examined 50 cases of systemic amyloidosis. In each case the type of amyloid was characterized by the current gold standard approach including an extensive clinical investigation for plasma cell disorders, serum and genetic testing for amyloidogenic variants of TTR, and immunohistochemistry for TTR, SAA, immunoglobulin kappa and lambda light chains, and SAP. The study included 16 TTR, 9 SAA and 25 AL amyloidosis. In this validation set, mass spectrometry identified the amyloid type with 100% specificity and sensitivity. The details of the study have been published and the citation for further reading is provided in this slide.
50 Cases of Amyloidosis3 |
Jump to section:
- Introduction
- Amyloidosis
- Diagnosis1
- Slide Images
- Subtypes: Historical Context
- Subtypes2
- Subtyping
- Subtyping
- Subtyping in Tissues
- Mass Spectrometry-Based Proteomic Analysis of Amyloidosis
- Protein Extraction
- Fragmented Peptides
- Mass/charge of Daughter Ions Measured
- Bioinformatics
- Case History 1
- Bone Marrow Biopsy Image
- Flow Cytometry Immunophenotyping
- Bone Marrow Image
- Congo Red-Positive Slide Image
- Immunohistochemistry Slide Images
- Microdissection
- MS-Based Proteomic Analysis
- Diagnosis
- Case History 2
- Mass Spectrometry Results in 50 Cases of Amyloidosis3
- Summary: Diagnosis
- Summary: Classification
- References
- Questions?
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