Diagnosis and Classification of Amyloidosis

MS-Based Proteomic Analysis

December 2009

This slide shows the results of mass spectrometry analysis performed on tryptic peptides obtained from 4 different microdissection samples (indicated as samples 1-4) and a negative blank control. The left column shows the list of proteins identified, and the numbers in each column indicate the number of spectra identified for each protein in each sample. In addition to proteins present in the glomerular microenvironment, the most abundant protein identified included serum amyloid P component (SAP), immunoglobulin lambda light chain constant region, and immunoglobulin lambda light chain variable region. This result indicates AL (lambda)-type amyloidosis.

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Proteomic Analysis

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• Introduction
• Amyloidosis
• Diagnosis¹
• Slide Images
• Subtypes: Historical Context
• Subtypes²
• Subtyping
• Subtyping
• Subtyping in Tissues
• Mass Spectrometry-Based Proteomic Analysis of Amyloidosis
• Protein Extraction
• Fragmented Peptides
• Mass/charge of Daughter Ions Measured
• Bioinformatics
• Case History 1
• Bone Marrow Biopsy Image
• Flow Cytometry Immunophenotyping
• Bone Marrow Image
• Congo Red-Positive Slide Image
• Immunohistochemistry Slide Images
• Microdissection
• MS-Based Proteomic Analysis
• Diagnosis
• Case History 2
• Renal Biopsy Image
• MS-Based Proteomic Analysis
• Diagnosis
• Mass Spectrometry Results in 50 Cases of Amyloidosis³
• Summary: Diagnosis
• Summary: Classification
• References
• Questions?