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Diagnosis and Classification of Amyloidosis

Renal Biopsy Image

Slide 25

December 2009

The renal biopsy showed amorphous material deposition in the glomeruli which was Congophilic and had characteristic features of amyloid under polarized light as shown in left upper and lower panels. As there were no glomeruli available in the frozen tissue sample for amyloid typing by immunofluorescence, mass spectrometry-based proteomic analysis was performed to identify the subtype of amyloid deposits. Congo red-stained sections were visualized under fluorescent light and areas giving characteristic red color were microdissected as shown in upper right panel. Lower right panel shows a single glomerulus microdissected for mass spectrometry analysis.

Renal Biopsy Image


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