Diagnosis and Classification of Amyloidosis
Renal Biopsy Image
December 2009
The renal biopsy showed amorphous material deposition in the glomeruli which was Congophilic and had characteristic features of amyloid under polarized light as shown in left upper and lower panels. As there were no glomeruli available in the frozen tissue sample for amyloid typing by immunofluorescence, mass spectrometry-based proteomic analysis was performed to identify the subtype of amyloid deposits. Congo red-stained sections were visualized under fluorescent light and areas giving characteristic red color were microdissected as shown in upper right panel. Lower right panel shows a single glomerulus microdissected for mass spectrometry analysis.
Renal Biopsy Image |
Jump to section:
- Introduction
- Amyloidosis
- Diagnosis1
- Slide Images
- Subtypes: Historical Context
- Subtypes2
- Subtyping
- Subtyping
- Subtyping in Tissues
- Mass Spectrometry-Based Proteomic Analysis of Amyloidosis
- Protein Extraction
- Fragmented Peptides
- Mass/charge of Daughter Ions Measured
- Bioinformatics
- Case History 1
- Bone Marrow Biopsy Image
- Flow Cytometry Immunophenotyping
- Bone Marrow Image
- Congo Red-Positive Slide Image
- Immunohistochemistry Slide Images
- Microdissection
- MS-Based Proteomic Analysis
- Diagnosis
- Case History 2
- Renal Biopsy Image
- MS-Based Proteomic Analysis
- Diagnosis
- Mass Spectrometry Results in 50 Cases of Amyloidosis3
- Summary: Diagnosis
- Summary: Classification
- References
- Questions?
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