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Monitoring Monoclonal Gammopathies

Free Light Chain: Antibody Specificity

Slide 13

November 2009

The introduction of the nephelometric assay to quantitate free light chains has provided a solution to this problem. The assay has specificity for free light chains and will not bind light chains that are complexed to heavy chains. That means that even in the presence of large amounts of polyclonal immunoglobulin, the assay can measure small amounts of the light chains. The complete assay is really 2 separate measurements to quantitate kappa free light chain and lambda free light chain concentrations and also to calculate the kappa:lambda free light chain ratio.

Antibody Specificity


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