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Risk Stratification in Chronic Lymphocytic Leukemia

The Role of the Clinical Laboratory



ZAP-70 in B-CLL: Detection

Slide 14

November 2008

The problem with ZAP-70 comes in some of the technical aspects in detecting this marker. It is certainly technically challenging within the laboratory. Various antibodies are available, and they all have some variation in their ability to detect ZAP-70. Since this is an intracellular marker, it requires making the cytoplasmic membrane permeable. Controls are needed to use to set both positive and negative staining and appropriate gating strategies must be employed.

Likewise, there are different methods analytically that have been used to calculate the percent positivity, thus a lot of problems have probably prevented this marker from being uniformly adapted in all flow cytometry laboratories.

ZAP-70 can also be detected by immunohistochemistry and tissue sections. However, this technique is certainly associated with a subjective interpretation and relatively few studies have been done showing its clinical utilization.

ZAP-70 in B-CLL: Detection

 


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