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Preparation of Platelet Poor Plasma for Special Coagulation Testing


Slide 19

October 2008

I have presented the procedure for preparing platelets poor plasma for special coagulation testing. If carefully followed we will have the best specimen possible from which your patient will get accurate, high-quality results.

In conclusion, the preprocessing risk factors for clotted specimens are as follows:

  • Traumatic venipuncture and the resulting tissue thromboplastin and hemolysis may activate the clotting cascade.
  • If a coagulation tube is drawn after a tube with a procoagulant, a clot activator, in it there is a hypothetical possibility for contamination and subsequent clotting.
  • If the specimen is not mixed the anticoagulant is not evenly mixed into the blood potentially allowing clots to form in the area absent of anticoagulant.
  • Too vigorous mixing may activate the platelets.
  • For hematocrits less than 25%, the plasma calcium is in excess proportion to sodium citrate anticoagulant, the plasma will be prone to clots and clotting time results will be falsely low.
  • A patient in a hypercoagulable state such as, which is DICICF, will be at risk for producing a clotted specimen.


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