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Antibodies Against Citrullinated Peptides

A Role in the Diagnosis of Rheumatoid Arthritis



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Development of Clinical Assays for Anti-Citrullinated Protein Antibodies

Slide 10

September 2008

As a result of the initial studies, development of clinical assays for anti-citrullinated protein antibodies focused on improving assay sensitivity while maintaining the high specificity. This was accomplished through optimization of protein or proteins used as a target antigen. During the course of this work, it was discovered that formation of a cyclic peptide improved the sensitivity of the assays.

To form these circularized structures, cystine residues were placed on either end of the peptide. When a disulfide bond was formed between the two cystine residues, a cyclic peptide resulted. This is where the term anticyclic citrullinated peptide antibody, or anti-CCP antibody, comes from. It is thought that the cyclic structure allows for maxim exposure of the citrullinated residue, thus improving analytic sensitivity.

Development of Clinical Assays

 


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