Free Light Chains & Monoclonal Gammopathies
Impact on Diagnostic Panels
Serum Protein Electrophoresis & Immunofixation Electrophoresis
August 2008
This slide is an example of a normal serum analyzed by protein electrophoresis or PEL and immunofixation electrophoresis or IFE. The upper portion of this slide is a picture of an agarose gel with the five fractions identified; albumin, Alpha 1, Alpha 2, Beta, and Gamma. Above the gel is the gel-scan or electropherogram, which allows us to quantitate each fraction.
The lower portion of the slide has a picture of an immunofixation gel. In this assay duplicate gel lanes are electrophoresed and then specific antisera is used to precipitate or immunofix the various immunoglobulin molecules in the agarose. We can see that all these molecules are distributed as smears rather then as discreet bands.
Serum Protein Electrophoresis |
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- Introduction
- Diagnostic Tests
- Mayo Clinic 1960-2003
- Plasma Cell Proliferative Disorders
- Serum Protein Electrophoresis & Immunofixation Electrophoresis
- Serum Protein Electrophoresis & Immunofixation Electrophoresis
- Monoclonal Serum Proteins: Newly Diagnosed Multiple Myeloma (n=1027)
- Serum Protein Electrophoresis & Immunofixation Electrophoresis
- Urine Protein Electrophoresis & Immunofixation Electrophoresis
- Intact Immunoglobulin/Free Light Chain
- FLC Reference Range
- Diagnoses for 428 Patients Selected for Urine Monoclonal Protein
- Serum PEL & IFE Results: 428 Pts with Urine Monoclonal Protein
- Laboratory Results: 428 Patients with Monoclonal Urinary Protein
- Potential Savings
- Uses of Serum Free Light Chain Assay
- Diagnostic Test Panel for Suspected Monoclonal Gammopathies
- Questions?
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