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Free Light Chains & Monoclonal Gammopathies

Impact on Diagnostic Panels

Serum Protein Electrophoresis & Immunofixation Electrophoresis

Slide 5

August 2008

This slide is an example of a normal serum analyzed by protein electrophoresis or PEL and immunofixation electrophoresis or IFE. The upper portion of this slide is a picture of an agarose gel with the five fractions identified; albumin, Alpha 1, Alpha 2, Beta, and Gamma. Above the gel is the gel-scan or electropherogram, which allows us to quantitate each fraction.

The lower portion of the slide has a picture of an immunofixation gel. In this assay duplicate gel lanes are electrophoresed and then specific antisera is used to precipitate or immunofix the various immunoglobulin molecules in the agarose. We can see that all these molecules are distributed as smears rather then as discreet bands.

Serum Protein Electrophoresis


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