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Supplemental Newborn Screening by MS/MS-Based Second-Tier Testing

What is Second-Tier Testing?

Slide 11

July 2008

What is second-tier testing? Second-tier testing is confirmatory testing which is performed when primary screening tests, either by tandem mass spectrometry or another method, yields an equivocal result. Basically, there is overlap of the normal control range and the disease range, so there is poor specificity. The confirmatory test measures the disease specific analyte or an analyte profile.

The normal second-tier test result then overrules primary screening results; the second-tier test utilizes the same specimen so there is no additional patient contact necessary.

What is Second-Tier Testing?


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