Laboratory Testing for Hepatitis C

Hepatitis C: Genotyping Assays

April 2008

In our patient, he was found to have an HCV RNA of 795,000 IU/mL. Because of the absence of clinical signs and symptoms, with the exception of an elevated serum, an alanine-transaminase level, we can conclude that he has chronic Hepatitis C.

The provider needs to know which additional tests should be requested to help guide specific anti-HCV therapy.

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Genotyping Assays

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• Introduction
• Patient Case
• Hepatitis C: Diagnostic Approach
• Acute Hepatitis C with Recovery
• Acute Hepatitis C with Progression to Chronic Infection
• Hepatitis C: Serologic Assays
• HCV Antigens Used in EIAs and CIAs for Detecting HCV Total Antibodies
• Patient Case
• Laboratory Algorithm
• Comparison of PCR, TMA, and bDNA
• Hepatitis C: Molecular Assays
• Hepatitis C: Molecular Tests
• Interpretation of Laboratory Test Results for HCV Infection
• Patient Case
• Hepatitis C: Genotyping Assays
• Patient Case
• Questions?