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HPV and p16 Testing in Oropharyngeal Squamous Cell Carcinoma

Methodology, Interpretation, and Significance

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March 2012

Human papillomavirus (HPV) is detected in 50% to 80% of oropharyngeal squamous cell carcinomas (OPSCCs). Dr. García describes the various testing methods for HPV detection, including DNA in situ hybridization (ISH), polymerase chain reaction (PCR), p16 immunochemistry, and E6/E7 messenger ribonucleic acid (mRNA). He also discusses the interpretation and significance of the assay results.


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