Flow Cytometry
The Flow Cytometry Laboratory performs several assays for clinical diagnostic testing. Major clinical applications include:
- Leukemia immunophenotyping by flow cytometry
(see #3287 “Leukemia/Lymphoma Immunophenotyping by Flow Cytometry”)
- Applicable to anticoagulated peripheral blood, bone marrow aspirates, tissues, or body fluids (for tissue, see #9439 “Leukemia/Lymphoma Immunophenotyping by Flow Cytometry, Tissue”)
- Useful for:
- Evaluating lymphocytoses of undetermined etiology
- Identifying B- and T-cell chronic lymphoproliferative disorders involving blood and bone marrow
- Distinguishing acute lymphoblastic leukemia (ALL) from acute myeloid leukemia (AML)
- Immunologic subtyping of ALL
- Distinguishing reactive lymphocytes/lymphoid hyperplasia from malignant lymphoma
- Distinguishing between malignant lymphoma and acute leukemia
- Phenotypic subclassification of B- and T-cell chronic lymphoproliferative disorders, including chronic lymphocytic leukemia, mantle cell lymphoma, and hairy cell leukemia
- Recognizing AML with minimal morphologic or cytochemical evidence of differentiation
- DNA ploidy analysis of solid tumor (see #9319
“DNA Ploidy of Solid Tumor by Flow Cytometry, Paraffin Block”)
- Applicable to formalin-fixed, paraffin-embedded tissue
- Useful for:
- Determining the prognosis for patients with certain tumors, including: prostate, colon, endometrial, and breast carcinoma
- Products of conception (triploidy)
- T- and B-cell quantitation (see #9336 “T- and
B-Cell Quantitation by Flow Cytometry” and #84348 “CD4 Count
for Monitoring, Blood”)
- Applicable to peripheral blood
- Quantitation of total T-cells, CD4 and CD8 subpopulations, B-cells, and NK cells
- Useful for:
- Determining immune status in a variety of acquired and inherited immunodeficiencies
- Monitoring total CD4 counts
- Monitoring CD3 populations following anti-CD3 therapy post-transplantation
- PI-linked antigen
- Detects the presence or absence of glycosylphosphatidylinositol-linked antigens on the surface of peripheral blood granulocytes, monocytes, and erythrocytes
- Useful in screening or confirming the diagnosis of paroxysmal nocturnal hemoglobinuria
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